Speculating on Immersive Cold Energy (ICE) Partitioned Fermentation for Fibre-based Feedstocks: process conception and biomolecular mechanisms.

 

The conception for fibre-based processed feedstock conversion to high-concentrate energy alcohols as in iso-pentenol and iso-butanol (the latter from ABE or acetone-butanol-ethanol, fermentation slanted towards isobutanol) is discussed here at length utilizing psychrophilic microbial cultures from the sub-Arctic (e. g. Seas or tundra) which would put an upper cap on maintenance energy needs during the frigid Winter months, and even during the "lighter" seasons, using cold saline or freshwater from lakes or rivers targeted for locations in Northern geographical areas like the NW Territories and N. Quebec prov./Labrador of Canada, Siberia and even the Baltic states near Soviet Russia.

 

The Model's Process Conception and Molecular Mechanisms.

 

The fundamental idea here on the topic we happened upon recently (and "brilliant" we might suggest) is to partition the cell's surrounding and interior milieus between the output in the outer milieu of the bioreactor where product can be partitioned out and cumulated to be downstream processed in the continuous reactor using microbial immobilization onto a column while the inside milieu of the cell is revved up metabolically to produce up-regulated rates of product through our new technology of PNA-B12 (there is also PNA-Ag as one other possibility at the moment) readily entering the cell through its molecular mechanism with the ATP-generating mitochondria and the enzymic catabolic network to form iso-pentenol or iso-butanol in the bioreactor's outer milieu together with transmembrane, outer membrane and mitochondrial membrane, modifications using mutational changes in the transport channeling porins of both mitochondria, for the export of energy-yielding currency or ATP, and the so-called dual-transporter carrier systems, at equilibrium, using facilitated diffusion (preferably rather than active transport that would utilize too much ATP energy by the cell) allowing the inner cell milieu to "vent" of what would otherwise be the overproduction of alcohols, and leading to toxification of the cell hosts.

 

Modification of Its Molecular Elements.

 

Mutational changes in enzymatic terms of transport proteins (porins) that somehow mutate fortuitously to accelerate rates of processing of substrate can be studied with proteomics and then "superposed" to genomic data for studying genetic mutations and then the genes edited onto the original genome with gene edited (GEditing) utilizing our same technology of PNA technology with exact precision.

 

There is also analysis using X-ray diffraction techniques to visualize the atomic centres and their bond lengths, and the orientations with respect to each other, shedding light on the fine-structural mechanisms of the reaction or transport processes. Remember, that when it comes to enzymic acceleration usually the matter centres around feed-back inhibition (FBI) which can be antagonized in mechanism as well as altering the Michaelis-Menten reaction describing receptor-substrate's binding on the outer and inner membrane's interphase in the cell.

 

Concluding Remarks.

 

What have we learned from this approach using this new form of biofuel fermentation or generation? Basically it marries together the idea of membrane biology and PNA-carrier based genetically regulated organismal (GRO class manipulations) of metabolism thus, in effect, partitioning the process between making "something on the spot" and on the other hand taking it out of the "manufacturing floor" rather than having it "back up", and thus, killing the cell host when in fact a faster rate of equilibrium can be achieved and maintained over the continuous, immobilized fermentor's reactive process. And, at that, at a lower temperature of maintenance at the reactor's floor and also within its insulated interior including its filtered solvent milieu.

 

It is presumed that distances between plant location where it is conveniently cold or cool, water sources, either saline or fresh, transport into the location of inputs including processed fibre feedstock and transport of downstream processed materials to their lower land depots for distribution be strategically located for cost and convenience.

 

There is now a belief that colder countries can utilize fibre-based feedstocks from farmlands for bioenergy fuel generation, and together with oil reserves and other energy survive independently from their more Southern neighbours who plan to up considerably their oil reserves through drilling like the U.S.A. without complicating trade ties to utilize fibrous feedstocks like pulp and even sugarcane bagasse although countries like the U.S.A. might opt for this also eventually.